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KMID : 0545120170270040838
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Journal of Microbiology and Biotechnology
2017 Volume.27 No. 4 p.838 ~ p.843
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High Efficiency Binding Aptamers for a Wide Range of Bacterial Sepsis Agents
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Graziani Ana Claudia
Stets Maria Isabel
Lopes Ana Luisa Kalb
Schluga Pedro Henrique Caires
Marton Soledad
Ferreira Ieda Mendes
de Andrade Antero Silva Ribeiro
Krieger Marco Aurelio
Cardoso Josiane
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Abstract
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Sepsis is a major health problem worldwide, with an extremely high rate of morbidity and mortality, partly due to delayed diagnosis during early disease. Currently, sepsis diagnosis requires bacterial culturing of blood samples over several days, whereas PCR-based molecular diagnosis methods are faster but lack sensitivity. The use of biosensors containing nucleic acid aptamers that bind targets with high affinity and specificity could accelerate sepsis diagnosis. Previously, we used the systematic evolution of ligands by exponential enrichment technique to develop the aptamers Antibac1 and Antibac2, targeting the ubiquitous bacterial peptidoglycan. Here, we show that these aptamers bind to four gram-positive and seven gram-negative bacterial sepsis agents with high binding efficiency. Thus, these aptamers could be used in combination as biological recognition elements in the development of biosensors that are an alternative to rapid bacteria detection, since they could provide culture and amplification-free tests for rapid clinical sepsis diagnosis.
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KEYWORD
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Anti-peptidoglycan aptamers, bacterial sepsis agents, qPCR assays, biosensor
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